Mitochondrial degradation by autophagy (mitophagy) in hepatocytes
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منابع مشابه
Mitochondrial degradation by autophagy (mitophagy) in GFP-LC3 transgenic hepatocytes during nutrient deprivation.
Fasting in vivo and nutrient deprivation in vitro enhance sequestration of mitochondria and other organelles by autophagy for recycling of essential nutrients. Here our goal was to use a transgenic mouse strain expressing green fluorescent protein (GFP) fused to rat microtubule-associated protein-1 light chain 3 (LC3), a marker protein for autophagy, to characterize the dynamics of mitochondria...
متن کامل3-MA 3-methyladenine CsA cyclosporin A JNK c-Jun N-terminal kinase LTR LysoTracker Red MTG MitoTracker Green MAPK mitogen-activated protein kinase MPT mitochondrial permeability transition PI3K phosphatidylinositol-3 kinase
Mitochondria become targets for autophagic degradation after nutrient deprivation, a process also termed mitophagy. In this study, we used LysoTracker Red (LTR) and MitoTracker Green to characterize the kinetics of autophagosomal proliferation and mitophagy in cultured rat hepatocytes. Autophagy induced by nutrient deprivation plus glucagon increased LTR uptake assessed with a fluorescence plat...
متن کاملAutophagy and mitophagy in cellular damage control☆
Autophagy and mitophagy are important cellular processes that are responsible for breaking down cellular contents, preserving energy and safeguarding against accumulation of damaged and aggregated biomolecules. This graphic review gives a broad summary of autophagy and discusses examples where autophagy is important in controlling protein degradation. In addition we highlight how autophagy and ...
متن کاملLysosomal Cholesterol Accumulation Sensitizes To Acetaminophen Hepatotoxicity by Impairing Mitophagy
The role of lysosomes in acetaminophen (APAP) hepatotoxicity is poorly understood. Here, we investigated the impact of genetic and drug-induced lysosomal cholesterol (LC) accumulation in APAP hepatotoxicity. Acid sphingomyelinase (ASMase)(-/-) mice exhibit LC accumulation and higher mortality after APAP overdose compared to ASMase(+/+) littermates. ASMase(-/-) hepatocytes display lower threshol...
متن کاملMitophagy.
Mitophagy describes the selective targeting and degradation of mitochondria by the autophagy pathway. In this process, defective mitochondria are first purged from the mitochondrial network then delivered to the lysosome by the autophagy machinery. Mitophagy has emerged as a key facet of mitochondrial quality control and has been implicated in a variety of human diseases. Disturbances in the ce...
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تاریخ انتشار 2008